is the method of separating and analyzing the
components of a chemical mixture; commonly used in the analysis of many
types of petroleum products. The test sample is
introduced into the chromatograph by means of an injection port,
which leads to a column inside an oven. In gas chromatography the
sample is volatilized and carried into the column by pressurized inert gas.
The sample components are absorbed into a liquid (called the stationary
phase) present inside the column. Regulating the oven temperature
causes the sample components to reach the detector at the column outlet in
order of respective boiling points. Each component is identified on the
basis of its retention time in the column. The data are shown on a
chromatogram in the form of peaks, whose heights represent the
concentration of the respective components. In liquid chromatography
(also called high-pressure normal phase liquid chromatography) the
sample is carried through the column by an inert liquid and passes
through a packing of tiny spheres coated with a stationary phase. The time
required for specific components to emerge from the column is directly
proportional to their degree of solubility in the stationary phase. Each
component is identified on the basis of its retention time in the column.
Also see clay/silica gel
analysis, ferrography,
infrared analysis,
mass spectrometer,
particle count,
spectrographic analysis.